cdna 195885 (Qiagen)

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Qiagen cdna 195885
Cdna 195885, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna+195885/Elution+Microtubes+CL/10__1128_slash_mcb__19__2__1479-31-37-51
Average 94 stars, based on 3 article reviews

cdna 195885 - by Bioz Stars, 2026-09

94/100 stars

Images

BAC Assay:

Article Title: Identification of a Gene That Reverses the Immortal Phenotype of a Subset of Cells and Is a Member of a Novel Family of Transcription Factor-Like Genes
Article Snippet: .. A 2.3-kb Nhe I band and a 7.5-kb Hin dIII band corresponding to cDNA 200901 from bacterial artificial chromosome (BAC) 526e7, a 4-kb Eco RI band corresponding to cDNA 231653 from BAC 526e7, and a 7.5-kb Hin dIII band corresponding to cDNA 195885 from BAC 316k7 were isolated by using the Qiaquick gel extraction kit (Qiagen) and cloned into the respective enzyme sites of pExSVNEO by standard ligation techniques. pExSVNEO was derived from pCMVex ( 34 ) by digestion with Spe I and Xba I, to remove the cytomegalovirus (CMV) promoter, and religation with a 38-bp oligonucleotide encoding the restriction sites Spe I, Apa I, Bgl II, Kpn I, Nhe I, Nru I, Sac II, and Xba I. ..

Article Title: Identification of a Gene That Reverses the Immortal Phenotype of a Subset of Cells and Is a Member of a Novel Family of Transcription Factor-Like Genes
Article Snippet: .. A 2.3-kb NheI band and a 7.5-kb HindIII band corresponding to cDNA 200901 from bacterial artificial chromosome (BAC) 526e7, a 4-kb EcoRI band corresponding to cDNA 231653 from BAC 526e7, and a 7.5-kb HindIII band corresponding to cDNA 195885 from BAC 316k7 were isolated by using the Qiaquick gel extraction kit (Qiagen) and cloned into the respective enzyme sites of pExSVNEO by standard ligation techniques. pExSVNEO was derived from pCMVex (34) by digestion with SpeI and XbaI, to remove the cytomegalovirus (CMV) promoter, and religation with a 38-bp oligonucleotide encoding the restriction sites SpeI, ApaI, BglII, KpnI, NheI, NruI, SacII, and XbaI. ..

Isolation:

Article Title: Identification of a Gene That Reverses the Immortal Phenotype of a Subset of Cells and Is a Member of a Novel Family of Transcription Factor-Like Genes
Article Snippet: .. A 2.3-kb NheI band and a 7.5-kb HindIII band corresponding to cDNA 200901 from bacterial artificial chromosome (BAC) 526e7, a 4-kb EcoRI band corresponding to cDNA 231653 from BAC 526e7, and a 7.5-kb HindIII band corresponding to cDNA 195885 from BAC 316k7 were isolated by using the Qiaquick gel extraction kit (Qiagen) and cloned into the respective enzyme sites of pExSVNEO by standard ligation techniques. pExSVNEO was derived from pCMVex (34) by digestion with SpeI and XbaI, to remove the cytomegalovirus (CMV) promoter, and religation with a 38-bp oligonucleotide encoding the restriction sites SpeI, ApaI, BglII, KpnI, NheI, NruI, SacII, and XbaI. ..

Gel Extraction:

Article Title: Identification of a Gene That Reverses the Immortal Phenotype of a Subset of Cells and Is a Member of a Novel Family of Transcription Factor-Like Genes
Article Snippet: .. A 2.3-kb NheI band and a 7.5-kb HindIII band corresponding to cDNA 200901 from bacterial artificial chromosome (BAC) 526e7, a 4-kb EcoRI band corresponding to cDNA 231653 from BAC 526e7, and a 7.5-kb HindIII band corresponding to cDNA 195885 from BAC 316k7 were isolated by using the Qiaquick gel extraction kit (Qiagen) and cloned into the respective enzyme sites of pExSVNEO by standard ligation techniques. pExSVNEO was derived from pCMVex (34) by digestion with SpeI and XbaI, to remove the cytomegalovirus (CMV) promoter, and religation with a 38-bp oligonucleotide encoding the restriction sites SpeI, ApaI, BglII, KpnI, NheI, NruI, SacII, and XbaI. ..

Clone Assay:

Article Title: Identification of a Gene That Reverses the Immortal Phenotype of a Subset of Cells and Is a Member of a Novel Family of Transcription Factor-Like Genes
Article Snippet: .. A 2.3-kb NheI band and a 7.5-kb HindIII band corresponding to cDNA 200901 from bacterial artificial chromosome (BAC) 526e7, a 4-kb EcoRI band corresponding to cDNA 231653 from BAC 526e7, and a 7.5-kb HindIII band corresponding to cDNA 195885 from BAC 316k7 were isolated by using the Qiaquick gel extraction kit (Qiagen) and cloned into the respective enzyme sites of pExSVNEO by standard ligation techniques. pExSVNEO was derived from pCMVex (34) by digestion with SpeI and XbaI, to remove the cytomegalovirus (CMV) promoter, and religation with a 38-bp oligonucleotide encoding the restriction sites SpeI, ApaI, BglII, KpnI, NheI, NruI, SacII, and XbaI. ..

Ligation:

Article Title: Identification of a Gene That Reverses the Immortal Phenotype of a Subset of Cells and Is a Member of a Novel Family of Transcription Factor-Like Genes
Article Snippet: .. A 2.3-kb NheI band and a 7.5-kb HindIII band corresponding to cDNA 200901 from bacterial artificial chromosome (BAC) 526e7, a 4-kb EcoRI band corresponding to cDNA 231653 from BAC 526e7, and a 7.5-kb HindIII band corresponding to cDNA 195885 from BAC 316k7 were isolated by using the Qiaquick gel extraction kit (Qiagen) and cloned into the respective enzyme sites of pExSVNEO by standard ligation techniques. pExSVNEO was derived from pCMVex (34) by digestion with SpeI and XbaI, to remove the cytomegalovirus (CMV) promoter, and religation with a 38-bp oligonucleotide encoding the restriction sites SpeI, ApaI, BglII, KpnI, NheI, NruI, SacII, and XbaI. ..

Derivative Assay:

Article Title: Identification of a Gene That Reverses the Immortal Phenotype of a Subset of Cells and Is a Member of a Novel Family of Transcription Factor-Like Genes
Article Snippet: .. A 2.3-kb NheI band and a 7.5-kb HindIII band corresponding to cDNA 200901 from bacterial artificial chromosome (BAC) 526e7, a 4-kb EcoRI band corresponding to cDNA 231653 from BAC 526e7, and a 7.5-kb HindIII band corresponding to cDNA 195885 from BAC 316k7 were isolated by using the Qiaquick gel extraction kit (Qiagen) and cloned into the respective enzyme sites of pExSVNEO by standard ligation techniques. pExSVNEO was derived from pCMVex (34) by digestion with SpeI and XbaI, to remove the cytomegalovirus (CMV) promoter, and religation with a 38-bp oligonucleotide encoding the restriction sites SpeI, ApaI, BglII, KpnI, NheI, NruI, SacII, and XbaI. ..

Review